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rocky mountain lab strain of mouse-adapted scrapie prions  (rocky mountain labs)

 
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    rocky mountain labs rocky mountain lab strain of mouse-adapted scrapie prions
    Rocky Mountain Lab Strain Of Mouse Adapted Scrapie Prions, supplied by rocky mountain labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse-adapted+prion+strains/rocky+mountain+strain+mouse+adapted+scrapie+brain+homogenate/pmc02885418-192-14-8
    Average 90 stars, based on 1 article reviews
    rocky mountain lab strain of mouse-adapted scrapie prions - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Strain diversity in neurodegenerative disease: an argument for a personalized medicine approach to diagnosis and treatment.
    Article Snippet: Instead, high-throughput screening is typically done using mouse-adapted prion strains that can propagate in cells, including RML, which was developed at Rocky Mountain Laboratories by passaging sheep scrapie in mice.



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    rocky mountain lab strain of mouse-adapted scrapie prions  (rocky mountain labs)
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    rml; mouse-adapted sheep prion strain prions scn2a  (rocky mountain labs)
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    Representative images of immunofluorescent staining of PrP (D18 antibody; red) and ThS (yellow). (A) Uninfected N2a and <t>ScN2a</t> cells with and without exposure to PK. Arrows indicate accumulations of misfolded PrP. (B) iPSC derived neurons from individuals carrying the E200K mutation following exposure to PK. (C) iPSC derived neurons from non-carriers stained following exposure to PK. (D) Quantification of the number of PrP and ThS positive puncta following exposure to PK from B and C. Non-carriers unrelated to the kindred are indicated in green, non-carriers in the kindred in blue and E200K carriers in red. p=0.0017 (BU + NC vs. E200K) and 0.02 (NC vs. E200K). n=fields observed. BU1 n=9; BU3 n=6, NC10 n=4; NC11 n=4; NC12 n=4; CJD2 n=4; CJD3 n=14; CJD4 n=3; CJD5 n=3; CJD6 n=9; CJD29 n=14; CJD40 n=12. Mean +/- SEM is plotted. Scale bars = 10 µm
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    Representative images of immunofluorescent staining of PrP (D18 antibody; red) and ThS (yellow). (A) Uninfected N2a and <t>ScN2a</t> cells with and without exposure to PK. Arrows indicate accumulations of misfolded PrP. (B) iPSC derived neurons from individuals carrying the E200K mutation following exposure to PK. (C) iPSC derived neurons from non-carriers stained following exposure to PK. (D) Quantification of the number of PrP and ThS positive puncta following exposure to PK from B and C. Non-carriers unrelated to the kindred are indicated in green, non-carriers in the kindred in blue and E200K carriers in red. p=0.0017 (BU + NC vs. E200K) and 0.02 (NC vs. E200K). n=fields observed. BU1 n=9; BU3 n=6, NC10 n=4; NC11 n=4; NC12 n=4; CJD2 n=4; CJD3 n=14; CJD4 n=3; CJD5 n=3; CJD6 n=9; CJD29 n=14; CJD40 n=12. Mean +/- SEM is plotted. Scale bars = 10 µm
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    Representative images of immunofluorescent staining of PrP (D18 antibody; red) and ThS (yellow). (A) Uninfected N2a and <t>ScN2a</t> cells with and without exposure to PK. Arrows indicate accumulations of misfolded PrP. (B) iPSC derived neurons from individuals carrying the E200K mutation following exposure to PK. (C) iPSC derived neurons from non-carriers stained following exposure to PK. (D) Quantification of the number of PrP and ThS positive puncta following exposure to PK from B and C. Non-carriers unrelated to the kindred are indicated in green, non-carriers in the kindred in blue and E200K carriers in red. p=0.0017 (BU + NC vs. E200K) and 0.02 (NC vs. E200K). n=fields observed. BU1 n=9; BU3 n=6, NC10 n=4; NC11 n=4; NC12 n=4; CJD2 n=4; CJD3 n=14; CJD4 n=3; CJD5 n=3; CJD6 n=9; CJD29 n=14; CJD40 n=12. Mean +/- SEM is plotted. Scale bars = 10 µm
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    Representative images of immunofluorescent staining of PrP (D18 antibody; red) and ThS (yellow). (A) Uninfected N2a and <t>ScN2a</t> cells with and without exposure to PK. Arrows indicate accumulations of misfolded PrP. (B) iPSC derived neurons from individuals carrying the E200K mutation following exposure to PK. (C) iPSC derived neurons from non-carriers stained following exposure to PK. (D) Quantification of the number of PrP and ThS positive puncta following exposure to PK from B and C. Non-carriers unrelated to the kindred are indicated in green, non-carriers in the kindred in blue and E200K carriers in red. p=0.0017 (BU + NC vs. E200K) and 0.02 (NC vs. E200K). n=fields observed. BU1 n=9; BU3 n=6, NC10 n=4; NC11 n=4; NC12 n=4; CJD2 n=4; CJD3 n=14; CJD4 n=3; CJD5 n=3; CJD6 n=9; CJD29 n=14; CJD40 n=12. Mean +/- SEM is plotted. Scale bars = 10 µm
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    Representative images of immunofluorescent staining of PrP (D18 antibody; red) and ThS (yellow). (A) Uninfected N2a and <t>ScN2a</t> cells with and without exposure to PK. Arrows indicate accumulations of misfolded PrP. (B) iPSC derived neurons from individuals carrying the E200K mutation following exposure to PK. (C) iPSC derived neurons from non-carriers stained following exposure to PK. (D) Quantification of the number of PrP and ThS positive puncta following exposure to PK from B and C. Non-carriers unrelated to the kindred are indicated in green, non-carriers in the kindred in blue and E200K carriers in red. p=0.0017 (BU + NC vs. E200K) and 0.02 (NC vs. E200K). n=fields observed. BU1 n=9; BU3 n=6, NC10 n=4; NC11 n=4; NC12 n=4; CJD2 n=4; CJD3 n=14; CJD4 n=3; CJD5 n=3; CJD6 n=9; CJD29 n=14; CJD40 n=12. Mean +/- SEM is plotted. Scale bars = 10 µm
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    Representative images of immunofluorescent staining of PrP (D18 antibody; red) and ThS (yellow). (A) Uninfected N2a and <t>ScN2a</t> cells with and without exposure to PK. Arrows indicate accumulations of misfolded PrP. (B) iPSC derived neurons from individuals carrying the E200K mutation following exposure to PK. (C) iPSC derived neurons from non-carriers stained following exposure to PK. (D) Quantification of the number of PrP and ThS positive puncta following exposure to PK from B and C. Non-carriers unrelated to the kindred are indicated in green, non-carriers in the kindred in blue and E200K carriers in red. p=0.0017 (BU + NC vs. E200K) and 0.02 (NC vs. E200K). n=fields observed. BU1 n=9; BU3 n=6, NC10 n=4; NC11 n=4; NC12 n=4; CJD2 n=4; CJD3 n=14; CJD4 n=3; CJD5 n=3; CJD6 n=9; CJD29 n=14; CJD40 n=12. Mean +/- SEM is plotted. Scale bars = 10 µm
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    Image Search Results


    Representative images of immunofluorescent staining of PrP (D18 antibody; red) and ThS (yellow). (A) Uninfected N2a and ScN2a cells with and without exposure to PK. Arrows indicate accumulations of misfolded PrP. (B) iPSC derived neurons from individuals carrying the E200K mutation following exposure to PK. (C) iPSC derived neurons from non-carriers stained following exposure to PK. (D) Quantification of the number of PrP and ThS positive puncta following exposure to PK from B and C. Non-carriers unrelated to the kindred are indicated in green, non-carriers in the kindred in blue and E200K carriers in red. p=0.0017 (BU + NC vs. E200K) and 0.02 (NC vs. E200K). n=fields observed. BU1 n=9; BU3 n=6, NC10 n=4; NC11 n=4; NC12 n=4; CJD2 n=4; CJD3 n=14; CJD4 n=3; CJD5 n=3; CJD6 n=9; CJD29 n=14; CJD40 n=12. Mean +/- SEM is plotted. Scale bars = 10 µm

    Journal: bioRxiv

    Article Title: An iPSC library from a family with Creutzfeldt-Jakob disease caused by the E200K mutation

    doi: 10.1101/2022.05.20.491674

    Figure Lengend Snippet: Representative images of immunofluorescent staining of PrP (D18 antibody; red) and ThS (yellow). (A) Uninfected N2a and ScN2a cells with and without exposure to PK. Arrows indicate accumulations of misfolded PrP. (B) iPSC derived neurons from individuals carrying the E200K mutation following exposure to PK. (C) iPSC derived neurons from non-carriers stained following exposure to PK. (D) Quantification of the number of PrP and ThS positive puncta following exposure to PK from B and C. Non-carriers unrelated to the kindred are indicated in green, non-carriers in the kindred in blue and E200K carriers in red. p=0.0017 (BU + NC vs. E200K) and 0.02 (NC vs. E200K). n=fields observed. BU1 n=9; BU3 n=6, NC10 n=4; NC11 n=4; NC12 n=4; CJD2 n=4; CJD3 n=14; CJD4 n=3; CJD5 n=3; CJD6 n=9; CJD29 n=14; CJD40 n=12. Mean +/- SEM is plotted. Scale bars = 10 µm

    Article Snippet: As a positive control, we used mouse neuroblastoma cells (N2a) that are chronically infected with Rocky Mountain Lab (RML; a mouse-adapted sheep prion strain) prions (ScN2a) ( ).

    Techniques: Staining, Derivative Assay, Mutagenesis

    Seeding activity is assessed using the Real Time-Quaking Induced Conversion (RT-QuIC) assay. (A) Sensitivity of the assay is demonstrated by a positive result using a 10 - 7 dilution of brain homogenate from a mouse at end-stage RML prion infection. n = 5 wells per sample. A 10 -4 dilution of uninfected, normal brain homogenate (NBH) is used as a negative control. (B) RT-QuIC is capable of detecting seeding activity in as little as 2 pg of total protein from lysates of chronically RML infected N2a cells (ScN2a). Uninfected N2a cell lysate is used as a negative control. n = 5 wells per sample. (C) No seeding activity was detected in lysates of 95 day post differentiation iPSC derived cortical neurons. n = 5 wells seeded with 2 ng protein per sample. All experiments were performed using full length (23-231) recombinant bank vole PrP. Mean thioflavin T fluorescence as a percentage of maximum signal is plotted against time in hours.

    Journal: bioRxiv

    Article Title: An iPSC library from a family with Creutzfeldt-Jakob disease caused by the E200K mutation

    doi: 10.1101/2022.05.20.491674

    Figure Lengend Snippet: Seeding activity is assessed using the Real Time-Quaking Induced Conversion (RT-QuIC) assay. (A) Sensitivity of the assay is demonstrated by a positive result using a 10 - 7 dilution of brain homogenate from a mouse at end-stage RML prion infection. n = 5 wells per sample. A 10 -4 dilution of uninfected, normal brain homogenate (NBH) is used as a negative control. (B) RT-QuIC is capable of detecting seeding activity in as little as 2 pg of total protein from lysates of chronically RML infected N2a cells (ScN2a). Uninfected N2a cell lysate is used as a negative control. n = 5 wells per sample. (C) No seeding activity was detected in lysates of 95 day post differentiation iPSC derived cortical neurons. n = 5 wells seeded with 2 ng protein per sample. All experiments were performed using full length (23-231) recombinant bank vole PrP. Mean thioflavin T fluorescence as a percentage of maximum signal is plotted against time in hours.

    Article Snippet: As a positive control, we used mouse neuroblastoma cells (N2a) that are chronically infected with Rocky Mountain Lab (RML; a mouse-adapted sheep prion strain) prions (ScN2a) ( ).

    Techniques: Activity Assay, Infection, Negative Control, Derivative Assay, Recombinant, Fluorescence